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KMID : 0861020020170020119
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Korea Journal of Herbology
2002 Volume.17 No. 2 p.119 ~ p.124
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Effect of Sophorae Radix on Methylmercury-Induced Myotoxicity in Cultured Myocardial Cells
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Abstract
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Objectives : To evaluate the toxic effect of methylmercuric chloride(MMC) and the protective effect of Sophorae Radix(SR) in cultured mouse myocardial cells, cytotoxicity was measured by MTT assay
Methods : Cultured myocardial cells were incubated for 72 hours in the media containing 5~40 ㎛ concentrations of MMC. And also, the protective effect of SR was determined by sulforhodamine B(SRB) for protein sythesis and radioactive assay for DNA sythesis in these cultures.
Results : Cell viability was significantly decreased in a dose-dependent manner after cultured myocardial cells were exposed to 20 ㎛ MMC for 72 hours. In the neuroprotective effect of SR on MMC-induced cytotoxicity, SR blocked the MMC-induced myotoxocity in these cultures.
Conclusion : It suggests that MMC is toxic against cultured mouse myocardial cells and SR is effective in blocking the meurotoxicity induced by MMC.
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